Thermocycler Calibration and Validation

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Thermocyclers are instruments used to amplify DNA and RNA samples by the polymerase chain reaction. The thermocycler raises and lowers the temperature of the samples in a holding block in discrete, pre-programmed steps, allowing for denaturation and reannealing of samples with various reagents. Amplified genetic material can be used in many downstream applications such as cloning, sequencing, expression analysis, and genotyping.

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To ensure your thermocycler raises and lowering temperature accurately, we perform the following calibrations:

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1. We check the temperature at 24 wells in 96-wells at the denaturation temperature of 92-95 Celsius.

2. We check the temperature of 24 wells in 96-wells block at the annealing stage of 50-55 Celsius.

3. We check the temperature of 24 wells in 96-wells block at the elongation stage of 65-70 Celsius.